Russian scientists have grown transgenic goat for tumor therapy

Biotechnology, undeniable breakthrough in the history of civilization, can and should be improved. First substances derived from biotechnology, extracted from simple organisms such as bacteria. However, bacteria, despite their attractive simplicity of the structure and breeding are very far from the man, and often proteins produced through them, not exactly correspond to the desired. Mammals in this context is much easier, because their cells protein synthesis system similar to that of humans. We are working on the creation of transgenic animals capable of producing the required hormones, enzymes or other proteins.

Scientists from Novosibirsk with Brazilian colleagues were transgenic goats capable of producing protein, stimulating hematopoiesis in the bone marrow, which is important in the rehabilitation of patients with cancerous tumors after removal. Animals are completely healthy and recovered a large amount of milk required protein.

  • The animal, which can ensure the future of thousands of cancer patients.  And with the mind - the goat as a goat.
  • The animal, which can ensure the future of thousands of cancer patients. And with the mind — the goat as a goat.

At the mention of the term "transgenic animal" often come to mind mice than goats. Indeed, the various strains of mice with artificially altered DNA now a great many, but almost all of them are used in scientific research and bringing more new information, but not commercial use. Keep out of the laboratory herd hoofed animals (and most laboratory animals for his life involved in only one series of experiments) — a dubious and expensive. But the breeding of transgenic goats for commercial use is much more convenient than the mouse: you can make it so that the protein of the animal will be excreted in milk and milk yield of goats is quite significant, can not be said about the little rodents.

 With transgenic goats Russian scientists began to work more than a decade ago, when the grown animal that gives milk to human lactoferrin. At this time, another group of scientists from Brazil and the Russian Institute of Molecular Genetics and Laboratory of Genetics, Institute of Cytology and Genetics, Russian Academy of Sciences set out to create goats that produce human granulocyte-colony stimulating factor (G-CSF).

Transgenic goats were obtained in Brazil at the Faculty of goat and sheep at the University of Ceara by direct DNA introduction of genetic engineering in the design of pronuclear (similar to the core) of fertilized eggs. In the future, these eggs were transplanted recipient hormonally stimulated goats. Injection of DNA into the pronucleus — presently unique method of producing transgenic animals.

This protein stimulates the bone marrow — the body that can produce new blood cells and immune system. Such stimulation is needed patients after surgery removal of a malignant tumor. When the last is cut, the patient is transplanted bone marrow cells that need to "force" to work to restore the immune system and the blood. Thanks to treatment with G-CSF bone marrow cells regenerate quickly in number, and less than a month hematopoiesis is completely back to normal. Koloniestimuliruschy factor can be entered and in radiation sickness, and other violations of blood.

The only drawback of this method of treatment — its high cost. A full course of treatment with G-CSF is now 6000-12000 U.S. dollars. The study showed that the use of transgenic goats can significantly reduce these costs. Oleg Serov, head of the Laboratory of Genetics Institute of Cytology and Genetics SB RAS, explains: "Simple calculations show that if the transgenic goat secretes human G-CSF in milk at 1mg/ml (or 1 gram per liter), with an average milk yield of dairy goats 200-300 liters per lactation potentially produced 200-300 grams of the desired protein. Of course, the allocation of G-CSF will be a loss of milk to 70%, but the output from a liter of milk can be expected to at least 300 mg. Therapeutic doses of G-CSF — from 5 to 10 mg for the full course. From this follows that a herd of transgenic goats heads 25-30 can provide from 5000 to 9000 liters per year, which potentially provides products 1,5-9 kg G-CSF, ie covers the needs of millions of patients. "

The team raised two transgenic animals, male and female, they are healthy and produce offspring. The study found that all of the clinical indicators of goats in the normal and the desired protein is found only in animal milk, but not in his blood (the latter could be bad for the health of goats and would mean that the expression of the gene is not as planned). Previous experiments on the production of animals secrete granulocyte-colony stimulating factor in transgenic mice were held at the Institute of Cytology and Genetics in Novosibirsk. Here in rodents were tested and all the necessary genetic engineering design.

Now that the method for producing transgenic animals that produce G-CSF developed, you can put the production of proteins for industrial, commercial basis. Specific agreements with biotech companies, scientists have not yet, but I want to believe that everything is still ahead and hopefully this project will find a practical embodiment, the benefit of which will be able to assess the real patients.

Source of information:

Moura RR, Albuquerque ES, Melo CH, Alcantara-Neto AS, Batista RI, Nunes-Pinheiro DC, Pereira AF, Teixeira DI, Melo LM, Serova IA, Andreeva LE, Serov OL, Freitas VJ, Dynamics of recombinant hG-CSF in transgenic goat: preliminary study in the founder during hormonally induced lactation. Animal Biotechnology, 24, 2013, 10-14.

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