Russian scientists a new way to prepare the trachea transplant

Specialists State of the Russian Cancer Research Center. NN Blokhin (RCRC) RAMS offered a new technique for the preparation of a donor trachea transplant. While she was tested on laboratory mice.

The trachea is a wide receiver, consisting of a 16-20 half-rings of cartilage covered with mucous membrane from the inside — epithelium. Certain infectious diseases, malignant tumors cause stenosis or injury, it is the compression of the trachea. In such cases, doctors removed the affected area and replace it with a prosthesis or a transplant. These procedures require extensive training and are often complicated by the reactions of the immune system — the rejection of the graft. It stimulates the epithelium of the trachea donor rejection. In other cases, the damaged cartilage. For example, a commonly used method by which the future graft repeatedly frozen to -70 ° C and then thawed. In this partially preserved mucosa, but the cartilage is destroyed — the basis of the trachea, which determines its structuring and elasticity.

GU Cancer Research Center scientists have tried to remove the donor tracheal epithelium, destroying it with sodium hypochlorite, and in the cartilage matrix of the trachea to move the stem cells of the recipient (ie the person to whom it will be transplanted).

A new method has been tested on laboratory mice of two different lines. Black line mouse C57BL / 6 mice served as donors white BALB / c — recipients. Donors were removed under anesthesia in the trachea and was treated for three days with a 5 percent solution of sodium hypochlorite (NaClO4). After this treatment, the cells died mucosa and cartilage cells (chondrocytes) remained intact.

Then the cartilage matrix populated with stem cells taken from the femurs of recipient mice. Two days later the stem cells were fixed on the surface of the cartilage tissue and differentiate into fibroblasts — connective tissue cells.

Scientists believe that fibroblasts may further participate in the formation of high-grade mucosa of the recipient’s own cells that do not cause it to immunological reactions. Chondrocytes donor also provoke graft rejection: obviously, because of the high density of the chondrocytes of cartilage tissue antigens almost no contact with the cells of the immune system of the new host.

Researchers believe that the proposed procedure would be promising for tracheal transplantation.

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