Antioxidants thiols used in many diseases, in combination with vitamin B12 have a prooxidant effect, furthermore, are toxic to cells and tissues. However, the toxic effect can be used in tumor therapy. Tissue Engineering Laboratory specialists
The work of scientists supported by analytical departmental target program "Development of Scientific Potential of Higher Education" and the Federal Target Program "Research and scientific-pedagogical personnel of innovative Russia" for 2009-2013.
Pharmaceutical preparations based on thiol compounds: N-acetylcysteine and glutathione is widely used in medicine for increasing the effectiveness of chemotherapy and radiotherapy and to reduce their side effects in the treatment of bronchial and lung diseases, diabetes, atherosclerosis and cataracts. Thiols inhibit the growth of some tumors and stimulate the immune system. Unfortunately, they do not mix well with vitamin B12, which is found in many multivitamin complexes, assigned patient for maintenance therapy. Co-administration of thiols and B12 may cause damage to organs and tissues, or at least to weaken the therapeutic effect. But this feature can be useful if you need to damage tumor cells.
Scientists have studied the sensitivity of tumor cells to the effects of various thiols and their combinations with vitamin B12. For the work they have chosen well studied human lymphocytic leukemia cell line HL60. In cell culture was added dithiothreitol, N-acetylcysteine, glutathione or different concentrations either alone or in conjunction with B12 and a day later the number of living cells was determined and compared with the untreated control.
It was found that the presence of thiol antioxidants to B12 are significantly more toxic to human lymphoid cells and induce apoptosis. Partially protect cells from death may compound deferoxamine, which binds free intracellular iron. Scientists note that the results should be considered in conjunction with the appointment of antioxidants vitamin complexes, especially at high doses.